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Pretreatment and characterization of CA The manually separated CA filters were blended and soaked in a 2% NaOH solution and subjected to three different TA pretreatments TA 30 C, TA 90 C, and TA 121 C incubated at different time periods 24 h, 1 h, and 20 min, respectively

In the second step, 7 l cDNA from step-1 was amplified using N gene forward primer (5-CACATTGGCACCCGCAATC-3), N gene reverse primer (5-GAGGAACGAGAAGAGGCTTG-3) and probe (5-FAM-ACTTCCTCAAGGAACAACATTGCCA-QSY-3) using Taqman mastermix (Thermo Fisher)

4 uses a San Andrs Negro Cultivo Tonto wrapper which is known for its unique characteristics since it is harvested using traditional methods such as how the Japanese harvest fruit

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